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Updated: Feb 10, 2026

Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
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Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells

Published on: July 29, 2016

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1α,25(OH)2-Vitamin D3 Inhibits C2C12 Cell Differentiation by Activating c-Src and ERK1/2

Zhonghua Wang, Aijun Jiang, Jingwei Mei

    Clinical Laboratory
    |May 9, 2018
    PubMed
    Abstract

    Insights

    1α,25(OH)2-vitamin D3 (1,25-D3) inhibits C2C12 cell differentiation and myotube fusion by activating Src and ERK1/2. Src acts upstream of ERK1/2 in this inhibitory pathway.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Endocrinology

    Background:

    • 1α,25(OH)2-vitamin D3 (1,25-D3), a steroid hormone, activates MAPK cascades in various cell types.
    • 1,25-D3 exhibits distinct roles during C2C12 cell proliferation, differentiation, and maturation.
    • Investigating 1,25-D3's impact on C2C12 myogenic differentiation is crucial.

    Purpose of the Study:

    • To determine the effect of 1,25-D3 on C2C12 myogenic differentiation.
    • To elucidate the role of ERK1/2 in 1,25-D3-induced differentiation of C2C12 cells.

    Main Methods:

    • C2C12 cells were induced to differentiate using 2% horse serum with 1,25-D3 added every two days for seven days.
    • Western blot analysis was employed to assess myosin heavy chain (MHC) protein levels and Src/ERK1/2 phosphorylation.
    • MEK inhibitor U0126 and Src inhibitor PP2 were utilized to probe signaling pathway relationships.

    Main Results:

    • 1,25-D3 inhibited C2C12 cell differentiation and myotube fusion.
    • This inhibition was mediated by the phosphorylation and activation of Src and ERK1/2.
    • Inhibition of ERK1/2 by U0126 or PP2 promoted C2C12 differentiation, while U0126 did not affect Src phosphorylation.

    Conclusions:

    • 1,25-D3 likely inhibits C2C12 differentiation via the Src/ERK1/2 signaling pathway.
    • Src functions as an upstream regulator in the signaling cascade initiated by 1,25-D3.
    • Targeting the Src/ERK1/2 pathway could modulate 1,25-D3's effects on muscle differentiation.

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