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M1 macrophage recruitment correlates with worse outcome in SHH Medulloblastomas

Chanhee Lee1, Joongyub Lee2, Seung Ah Choi1

  • 1Division of Pediatric Neurosurgery, Seoul National University Children's Hospital, 101 Daehakro, Jongno-gu, 110-744, Seoul, Republic of Korea.

BMC Cancer
|May 10, 2018
PubMed
Abstract

Insights

In medulloblastoma, high M1 macrophage levels correlate with worse outcomes in the SHH subgroup. This finding suggests M1 macrophages, not M2, are key indicators of prognosis in SHH medulloblastoma.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Medulloblastoma (MB) comprises four molecular subgroups: WNT, SHH, Group 3, and Group 4.
  • Tumor-associated macrophages (TAMs) are key components of the tumor microenvironment, but their M1/M2 polarized states' roles in MB are unclear.

Purpose of the Study:

  • To investigate the correlation between TAM recruitment within medulloblastoma subgroups and patient prognosis.
  • To determine the differential impact of M1 and M2 macrophage phenotypes on outcomes in SHH medulloblastoma.

Main Methods:

  • Molecular subgrouping of MB tissues using a nanoString-based RNA assay.
  • Quantification of M1 and M2 TAMs via immunohistochemistry (IHC) and immunofluorescence (IF).
  • Survival analysis using Kaplan-Meier curves and Cox regression on clinical and macrophage data.

Main Results:

  • TAM recruitment was significantly higher in the SHH medulloblastoma subgroup compared to others.
  • High M1 TAM expression in SHH MB was associated with significantly worse overall survival (OS) and progression-free survival (PFS).
  • Relative risk for worse OS and PFS was notably high (11.918 and 6.022, respectively) in patients with high M1 expression.

Conclusions:

  • The M1 macrophage phenotype, not M2, is strongly associated with poorer outcomes in SHH medulloblastoma.
  • M1 TAM levels serve as a significant prognostic biomarker for SHH medulloblastoma.
Keywords:
MacrophageMedulloblastomaPrognosisRecruitmentSonic hedgehog

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