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Updated: Feb 10, 2026

Probiotic Studies in Neonatal Mice Using Gavage
Published on: January 27, 2019
Multispecies probiotics combination prevents ovalbumin-induced airway hyperreactivity in mice
Jui-Cheng Chen1, Cheng-Chih Tsai1, Chang-Chi Hsieh2
1Department of Food Science and Technology, HungKuang University, Taichung, Taiwan.
Background:
Probiotics could be beneficial to health and some of them have shown to modulate immune responses.
Aim:
The aim of this study is to investigate if the probiotic strains including Lactobacillus and Pediococcus strains are able to alleviate allergic reactions in an ovalbumin-induced airway allergy model.
Methods:
Lactobacillus multi-species preparation (LMP) was gavaged to BALB/c for total six weeks and BALB/c was challenged with ovalbumin in the last two weeks. A barometric whole-body plethysmography was used to assess enhanced pause (Penh) of airway hyperreactivity (AHR). Immunoglobulins (Ig) such as IgE, IgG1, IgG2a and cytokines such as IL-12, IFN-γ, IL-4, IL-5, TNF-α and IL-13 in bronchoalveolar lavage fluid were assayed using ELISA kits.
Results:
The results showed this LMP significantly reduced Th2 cytokines and enhanced Th1 cytokines production. OVA-specific IgE and IgG1 was lower in the probiotics-treated mice whereas IgG2a was increased. Most importantly, this murine model showed LMP supplementation significantly reduced AHR.
Conclusions:
Overall, this Lactobacillus multi-species preparation seemed to suppress OVA-sensitized airway hyperreactivity, thus serving as a possible candidate for therapeutic uses for allergic airway symptoms.
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