Related Experiment Video
Updated: Feb 10, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Enzymatic extract containing lignin peroxidase immobilized on carbon nanotubes: Potential biocatalyst in dye
Sabrina Feliciano Oliveira1, José Maria Rodrigues da Luz2, Maria Catarina Megumi Kasuya2
1Department of Microbiology, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha, 31270-901 Belo Horizonte, Minas Gerais, Brazil.
Abstract:
The majority of the textile dyes are harmful to the environment and potentially carcinogenic. Among strategies for their exclusion, the treatment of dye contaminated wastewater with fungal extract, containing lignin peroxidase (LiP), may be useful. Two fungi isolates, Pleurotus ostreatus (PLO9) and Ganoderma lucidum (GRM117), produced the enzymatic extract by fermentation in the lignocellulosic residue, Jatropha curcas seed cake. The extracts from PLO9 and GRM117 were immobilized on carbon nanotubes and showed an increase of 18 and 27-fold of LiP specific activity compared to the free enzyme. Also, LiP from both fungi extracts showed higher Vmax and lower Km values. Only the immobilized extracts could be efficiently reused in the dye decolourization, contrary, the carbon nanotubes became saturated and they should be discarded over time. This device may offer a final biocatalyst with higher catalytic efficiency and capability to be reused in the dye decolourization process.
More Related Videos
Related Concept Videos
The Carbon Cycle
Carbon Skeletons
Potential Energy
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
Carbonation Shrinkage
The concrete's permeability is slightly reduced as calcium carbonate produced during the reaction fills its pores. Furthermore, its strength is slightly enhanced as the water released during the reaction...
Carbon-dioxide Fixation
Standard Electrode Potentials

