Related Experiment Video

Updated: Feb 10, 2026

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
07:17

Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes

Published on: December 16, 2022

4.1K

Determination of Oocyte-Manipulation, Zygote-Manipulation, and Genome-Reprogramming Effects on the Transcriptomes of

Byungkuk Min1, Jung S Park1, Yong-Kook Kang1

  • 1Development and Differentiation Research Center, Korea Research Institute of Bioscience Biotechnology, Daejeon, South Korea.

Frontiers in Genetics
|May 10, 2018
PubMed

Insights

Somatic cell nuclear transfer (scNT) embryos face developmental issues due to manipulation and poor reprogramming. This study introduces sham nuclear-transfer (shNT) embryos to distinguish these effects, revealing distinct molecular signatures for manipulation versus genome reprogramming in cloned bovine embryos.

Area of Science:

  • Reproductive Biology and Embryology
  • Genomics and Transcriptomics
  • Biotechnology and Bioengineering

Background:

  • Somatic cell nuclear transfer (scNT) embryos exhibit developmental defects attributed to micro-operation damage and incomplete genome reprogramming.
  • Distinguishing the molecular impact of manipulation versus reprogramming is crucial for advancing scNT technologies.
  • Current methods lack the precision to isolate these two confounding factors in scNT embryos.

Purpose of the Study:

  • To develop and validate a novel bovine sham nuclear-transfer (shNT) embryo model.
  • To differentiate transcriptomic signatures associated with zygote manipulation from those linked to donor genome reprogramming in scNT embryos.
  • To identify molecular markers for improving scNT efficiency and evaluating reprogramming tools.

Main Methods:

  • Generation of bovine shNT blastocysts using standard nuclear transfer procedures but with an intact genome.
  • Transcriptomic profiling of shNT blastocysts compared against in-vitro fertilization (IVF) and scNT blastocysts.
  • Differential gene expression analysis, correlation analysis, and Gene Ontology (GO) enrichment analysis.

Main Results:

  • shNT blastocysts exhibited a unique transcriptomic profile, distinct from both IVF and scNT embryos.
  • Manipulation-associated differentially expressed genes (MADs) were common to shNT and scNT, while donor genome-associated DEGs (DADs) were specific to scNT.
  • Zygote manipulation-associated DEGs (zMADs) were identified as specific to shNT, with distinct GO terms related to homeostasis, ER stress, and protein transport.

Conclusions:

  • The shNT model successfully isolates the effects of zygote manipulation from genome reprogramming in scNT embryos.
  • Distinct transcriptomic signatures, particularly zMADs, provide insights into manipulation-induced molecular changes.
  • These findings offer a foundation for understanding and improving the molecular events governing cloned embryo development and reprogramming.
Keywords:
RNA-seqSCNTbovine embryogene expression profilingmanipulationnuclear transferreprogrammingstemness gene

More Related Videos

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation09:05

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Published on: December 25, 2017

13.0K
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish10:39

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Published on: April 22, 2017

11.8K

Related Concept Videos

Manipulation and Analysis01:21

Manipulation and Analysis

GIS manipulation and analysis functions are vital for decision-making and planning. These activities range from data retrieval tasks, such as selecting information based on specific criteria, to advanced analytical techniques that address complex spatial problems.One critical GIS analysis method is overlaying, which combines multiple data layers to examine impacts. For example, overlaying a river-dammed lake boundary with road networks can identify affected infrastructure. Another common...
302
Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.9K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.8K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.3K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.2K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K