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Updated: Feb 10, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
Prospects for engineering dynamic CRISPR-Cas transcriptional circuits to improve bioproduction
Jason Fontana1, William E Voje1,2, Jesse G Zalatan3,4
1Molecular Engineering and Sciences Institute and Center for Synthetic Biology, University of Washington, Seattle, WA, 98195, USA.
Abstract:
Dynamic control of gene expression is emerging as an important strategy for controlling flux in metabolic pathways and improving bioproduction of valuable compounds. Integrating dynamic genetic control tools with CRISPR-Cas transcriptional regulation could significantly improve our ability to fine-tune the expression of multiple endogenous and heterologous genes according to the state of the cell. In this mini-review, we combine an analysis of recent literature with examples from our own work to discuss the prospects and challenges of developing dynamically regulated CRISPR-Cas transcriptional control systems for applications in synthetic biology and metabolic engineering.
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