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Immune complex acute necrotizing glomerulonephritis with progression to diffuse glomerulosclerosis. A murine model.
S S Iskandar1, D R Gifford, S N Emancipator
1Case Western Reserve University, Cleveland, Ohio.
Summary
Male mice injected with horse-spleen apoferritin developed glomerulonephritis. Discontinuing injections led to glomerulosclerosis (GS) in survivors, offering a model for studying immune complex-mediated GS.
Area of Science:
- Nephrology
- Immunology
- Pathology
Background:
- Horse spleen apoferritin induces glomerulonephritis in mice.
- Glomerulosclerosis (GS) is a significant cause of kidney disease.
Purpose of the Study:
- To investigate the long-term effects of antigen withdrawal on glomerulonephritis.
- To establish a reproducible model for studying glomerulosclerosis.
Main Methods:
- Male BALB/c mice received daily intraperitoneal injections of horse-spleen apoferritin.
- Antigen injections were discontinued in surviving mice.
- Kidney tissues were examined 4 months post-treatment for glomerulosclerosis and tubulointerstitial damage.
Main Results:
- Mice developing glomerulonephritis showed extensive glomerulosclerosis (GS) after antigen withdrawal.
- Segmental GS affected over 40% of glomeruli in 10 out of 13 mice.
- Ultrastructural analysis revealed mesangial expansion due to matrix deposition, obliterating glomerular architecture.
Conclusions:
- Discontinuation of horse-spleen apoferritin in mice with induced glomerulonephritis leads to glomerulosclerosis (GS).
- This model is suitable for studying the pathogenesis of immune complex-mediated GS and cellular interactions involved.