Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hydrogen Bonds00:26

Hydrogen Bonds

134.3K
Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
134.3K
Hydrogen Bonds01:04

Hydrogen Bonds

15.0K
A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
15.0K
IR Spectrum Peak Broadening: Hydrogen Bonding01:23

IR Spectrum Peak Broadening: Hydrogen Bonding

1.9K
The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1.
However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular...
1.9K
Valence Bond Theory02:45

Valence Bond Theory

50.3K
Overview of Valence Bond Theory
50.3K
Covalent Bonds01:29

Covalent Bonds

163.7K
Overview
163.7K
Bond Energies and Bond Lengths02:49

Bond Energies and Bond Lengths

31.6K
Stable molecules exist because covalent bonds hold the atoms together. The strength of a covalent bond is measured by the energy required to break it, that is, the energy necessary to separate the bonded atoms. Separating any pair of bonded atoms requires energy — the stronger a bond, the greater the energy required to break it.
31.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A High-Throughput Platform for Measuring and Predicting Vitrification Behavior in Multicomponent Aqueous Solutions.

ACS applied materials & interfaces·2026
Same author

Chain Length as a Molecular Determinant of Hydrogen-Bond Dynamics in Biocondensates.

The journal of physical chemistry letters·2026
Same author

Solvent Reorganization in Stabilized Protein-Polymer Conjugates Visualized by Two-Dimensional Infrared and Nuclear Magnetic Resonance Spectroscopy.

JACS Au·2026
Same author

Low-cost calculation and analysis of 2D IR spectra of model diiron trinitrosyl complexes in the NO stretch region with vibrational perturbation theory.

Physical chemistry chemical physics : PCCP·2026
Same author

Membrane Composition Reshapes the Folding Landscape of a pH-Responsive Peptide.

The journal of physical chemistry letters·2025
Same author

Machine learning potentials accurately reproduce vibrational dynamics in complex environments.

The Journal of chemical physics·2025
Same journal

Divergent Aggregation Pathways of DNA-AuNPs: Non-Watson-Crick Assembly Mediated by Structurally Diverse Electrolytes.

The journal of physical chemistry. B·2026
Same journal

Assessing Fluoroacetate Defluorination Potential across Diverse Enzymes Using Quantum Chemistry.

The journal of physical chemistry. B·2026
Same journal

Na<b><sup>+</sup></b> Solvation and Association in Na(SO<sub>3</sub>CF<sub>3</sub>)-Dimethoxyethane Electrolytes by Large-Angle X-Ray Scattering and DFT Calculations.

The journal of physical chemistry. B·2026
Same journal

Donor-Acceptor Separation Augments Temperature Dependence of Kinetic Isotope Effects in NADH Model Hydride Transfer Reactions: Mimicking Enzyme versus Mutant Dynamics.

The journal of physical chemistry. B·2026
Same journal

Disordered Worm-Like Clusters in a Hexagonal Mesophase Former: Simulation and Thermodynamic Description.

The journal of physical chemistry. B·2026
Same journal

Comparative Biophysical Analysis of Healthy and Inflamed Intestinal Membrane Models Using Langmuir Monolayers.

The journal of physical chemistry. B·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Hydrogen Charging of Aluminum using Friction in Water
07:50

Hydrogen Charging of Aluminum using Friction in Water

Published on: January 28, 2020

6.6K

Crowding Stabilizes DMSO-Water Hydrogen-Bonding Interactions.

Kwang-Im Oh1, Carlos R Baiz1

  • 1Department of Chemistry , University of Texas at Austin , 105 E 24th St. Stop A5300 , Austin , TX 78712 , United States.

The Journal of Physical Chemistry. B
|May 11, 2018
PubMed
Summary
This summary is machine-generated.

Molecular crowding affects water structure and hydrogen bonding with dimethyl sulfoxide (DMSO). Amide additives enhance DMSO-water interactions, influencing solvation shells and biomolecular stability.

More Related Videos

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

7.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

Hydrogen Charging of Aluminum using Friction in Water
07:50

Hydrogen Charging of Aluminum using Friction in Water

Published on: January 28, 2020

6.6K
Controlling the Size, Shape and Stability of Supramolecular Polymers in Water
16:24

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Published on: August 2, 2012

19.3K
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
08:20

In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Published on: May 1, 2020

7.2K

Area of Science:

  • Physical Chemistry
  • Biophysics
  • Computational Chemistry

Background:

  • Intracellular water is confined by macromolecular crowding, impacting biomolecular interactions.
  • The role of crowding and heterogeneity in additive effects on biomolecular structure is poorly understood.

Purpose of the Study:

  • To elucidate how molecular crowding modulates hydrogen bonding between water and dimethyl sulfoxide (DMSO).
  • To investigate the mechanisms of solvation shell changes induced by amide crowders.

Main Methods:

  • Infrared spectroscopy to analyze hydrogen bonding.
  • Molecular dynamics simulations to model molecular interactions and solvation structures.

Main Results:

  • Formamide and dimethylformamide (DMF) increased S═O hydrogen bond populations in aqueous DMSO mixtures.
  • Additives enhanced water in the DMSO solvation shell by stabilizing DMSO-water bonds and destabilizing DMSO-DMSO interactions.
  • Hydrogen bond enthalpies: DMSO-water (61 kJ/mol) > DMSO-formamide (32 kJ/mol) > water-water (23 kJ/mol) ≫ formamide-water (4.7 kJ/mol).
  • DMSO induced amide dehydration due to strong DMSO-water interactions.

Conclusions:

  • Molecular crowding significantly alters water-cosolvent hydrogen bonding networks.
  • The observed DMSO-water interactions provide insights into mechanisms of protein destabilization by cosolvents.