Impact of Saliva Collection and Processing Methods on Aspartate Aminotransferase, Creatin Kinase and Lactate Dehydrogenase Activities
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Published on: June 21, 2018
Tomás Barranco1, Jose J Cerón1, Pía López-Jornet1
1Interdisciplinary Laboratory, Interlab-UMU, Campus of Excellence Mare Nostrum, University of Murcia.
Abstract:
We aimed to investigate the impact of saliva collection and processing methods on AST, CK and LDH. Saliva was collected from 17 healthy participants by a passive drool. Each saliva sample was distributed into 3 aliquots: not treated, centrifuged, and passed through cotton. Centrifugation improved the precision of assays and produced lower values of AST and CK. The use of cotton resulted in decreased levels of LDH. This data stress the importance of the standardization of sample processing to measure enzymes in saliva.
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