Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Size01:22

Cell Size

130.2K
Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.
Surface Area
Cells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding...
130.2K
Synaptic Signaling01:12

Synaptic Signaling

79.8K
Neurons communicate at synapses, or junctions, to excite or inhibit the activity of other neurons or target cells, such as muscles. Synapses may be chemical or electrical.
79.8K
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.7K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.7K
Chemistry of the Cell02:58

Chemistry of the Cell

48.3K
The cell is chemically composed of water, organic molecules and inorganic ions.
Water
The polarity of the water molecule and its resulting hydrogen bonding makes water a unique substance with special properties that are intimately tied to the processes of life. Life originally evolved in an aqueous environment, and most of an organism’s cellular chemistry and metabolism occur inside the aqueous contents of the cell’s cytoplasm. Special properties of water are its high heat capacity...
48.3K
Hair Cells01:22

Hair Cells

45.1K
Hair cells are the sensory receptors of the auditory system—they transduce mechanical sound waves into electrical energy that the nervous system can understand. Hair cells are located in the organ of Corti within the cochlea of the inner ear, between the basilar and tectorial membranes. The actual sensory receptors are called inner hair cells. The outer hair cells serve other functions, such as sound amplification in the cochlea, and are not discussed in detail here.
45.1K
What are Lipids?01:38

What are Lipids?

221.0K
Overview
221.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A participatory photovoice investigation of community assets, barriers, and opportunities to curb the opioid epidemic.

Journal of substance use and addiction treatment·2025
Same author

Personalised anaesthesia: three-dimensional printing of facial prosthetic for facial deformity with difficult airway.

British journal of anaesthesia·2018
Same author

The influence of ultra-pasteurization by indirect heating versus direct steam injection on skim and 2% fat milks.

Journal of dairy science·2017
Same author

Short communication: The effect of raw milk cooling on sensory perception and shelf life of high-temperature, short-time (HTST)-pasteurized skim milk.

Journal of dairy science·2016
Same author

A fenestrated aortic valve contributing to iatrogenic aortic insufficiency post mitral valve replacement.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Membrane biophysics define neuron and astrocyte progenitors in the neural lineage.

Stem cells (Dayton, Ohio)·2013

Related Experiment Video

Updated: Feb 10, 2026

Lipid Exchange Assay in Living Cells
08:59

Lipid Exchange Assay in Living Cells

Published on: March 21, 2025

1.1K

Cell-sized lipid vesicles for cell-cell synaptic therapies.

D Vallejo1, S H Lee1, D Lee2

  • 1Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Science Il, Irvine, California 92697, USA.

Technology
|May 11, 2018
PubMed
Summary
This summary is machine-generated.

Storing fragile cell-sized lipid vesicles in a double emulsion precursor extends their shelf life to over 8 months. This method enables stable storage and on-demand conversion for therapeutic and artificial cell applications.

Keywords:
Artificial CellsDewettingDouble EmulsionsDroplet MicrofluidicsGiant Unilamellar Vesicles

More Related Videos

Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells
08:37

Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

Published on: March 10, 2023

3.5K
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

13.0K

Related Experiment Videos

Last Updated: Feb 10, 2026

Lipid Exchange Assay in Living Cells
08:59

Lipid Exchange Assay in Living Cells

Published on: March 21, 2025

1.1K
Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells
08:37

Author Spotlight: Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

Published on: March 10, 2023

3.5K
Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction
07:33

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting LIMACS: a Novel Method to Analyze Protein-lipid Interaction

Published on: April 26, 2011

13.0K

Area of Science:

  • Biotechnology
  • Materials Science
  • Cell Biology

Background:

  • Cell-sized lipid vesicles (CLVs), including giant unilamellar vesicles (GUVs) and single-compartment multisomes (SCMs), are promising for therapeutics and artificial cells.
  • Their widespread adoption is limited by fragility and short shelf life.

Purpose of the Study:

  • To develop a method for long-term storage of CLVs.
  • To investigate interfacial parameters governing CLV formation from precursors.
  • To demonstrate therapeutic applications of CLVs.

Main Methods:

  • CLVs were stored in a double emulsion precursor form.
  • Interfacial parameters influencing morphological changes were analyzed.
  • CLVs presenting neuroligin-2 were used to interact with pancreatic β-cells.

Main Results:

  • The double emulsion precursor allowed storage of CLVs for at least 8 months.
  • Stored precursors could be converted into GUVs or SCMs on demand.
  • Neuroligin-2 presenting CLVs formed cell-cell synapses with pancreatic β-cells, stimulating insulin secretion and growth.

Conclusions:

  • Storing CLVs in double emulsion precursors overcomes shelf-life limitations.
  • This approach facilitates the use of CLVs in therapeutic applications.
  • CLVs can be engineered to form functional cell-cell interactions for therapeutic benefit.