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Updated: Feb 10, 2026
09:56
Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications
Published on: December 8, 2015
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Bioactivation of titanium dioxide scaffolds by ALP-functionalization
A Sengottuvelan1, P Balasubramanian1, J Will1
1Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstraße 6, 91058 Erlangen, Germany.
Bioactive Materials
|May 11, 2018
Summary
Functionalizing titanium dioxide scaffolds with alkaline phosphatase (ALP) enhances their bioactivity. This biomolecule coating promotes hydroxyapatite formation, making these scaffolds more suitable for bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Ceramic Engineering
Background:
- Three-dimensional titanium dioxide (TiO2) scaffolds offer biocompatibility and mechanical strength for bone tissue engineering.
- Their bioactivity is limited compared to materials like bioactive glass or hydroxyapatite (HA).
- Surface functionalization with biomolecules can improve scaffold bioactivity.
Purpose of the Study:
- To investigate the functionalization of 3D TiO2 scaffolds with alkaline phosphatase (ALP).
- To assess the impact of ALP on the bioactivity and hydroxyapatite formation of TiO2 scaffolds.
Main Methods:
- Fabrication of robust 3D TiO2 scaffolds using the foam replica method.
- Surface functionalization via dip-coating in a polydopamine/ALP solution.
- Confirmation of ALP presence through contact angle and enzymatic activity assays.
- Evaluation of hydroxyapatite formation in simulated body fluid (SBF) over 28 days.
Main Results:
- Successfully produced TiO2 scaffolds with a compressive strength of approximately 2.7 ± 0.3 MPa.
- Indirect confirmation of ALP presence on the scaffold surface.
- ALP-coated scaffolds demonstrated increased hydroxyapatite formation after 28 days in SBF compared to uncoated scaffolds.
Conclusions:
- Alkaline phosphatase enhances the bioactivity of titanium dioxide scaffolds.
- ALP functionalization transforms inert TiO2 into a promising bioactive system for bone tissue engineering.
- This approach offers a strategy to improve the efficacy of ceramic-based bone TE scaffolds.
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