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Updated: Feb 10, 2026

Rapid Generation of Amyloid from Native Proteins In vitro
Published on: December 5, 2013
ATR-FTIR Analysis of Amyloid Proteins
Jean-Marie Ruysschaert1, Vincent Raussens2
1Faculty of Science, Laboratory of Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles, Brussels, Belgium.
Abstract:
Attenuated total reflection FTIR (ATR-FTIR) has been used for decades to study protein secondary structures. More recently, it reveals also to be an exquisite and sensitive tool to study and discriminate amyloid aggregates. Based on the analysis of specific spectral features of β-sheet structures, we present here a detailed protocol to differentiate oligomers vs. fibrils. This protocol, applicable to all amyloid proteins, demonstrates the power of this inexpensive, rapid, and low protein material-demanding method.
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