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Circulating lncRNA IFNG-AS1 expression correlates with increased disease risk, higher disease severity and elevated

Yahuan Xu1, Bibo Shao2

  • 1Department of Cardiothoracic Surgery, Huangshi Central Hospital, Edong Healthcare Group, Affiliated Hospital of Hubei Polytechnic University, Huangshi, China.

Journal of Clinical Laboratory Analysis
|May 11, 2018
PubMed

Insights

Long, non-coding RNA IFNG-AS1 is upregulated in coronary artery disease (CAD) patients, correlating with disease risk, severity, and inflammation. Other tested lncRNAs showed no significant associations.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Research

Background:

  • Coronary artery disease (CAD) poses a significant global health burden.
  • Understanding the molecular mechanisms underlying CAD is crucial for developing effective diagnostic and therapeutic strategies.
  • Long non-coding RNAs (lncRNAs) are emerging as key regulators in various diseases, including cardiovascular conditions.

Purpose of the Study:

  • To investigate the association of circulating long, non-coding RNA (lncRNA) IFNG-AS1, lncRNA ANRIL, and lncRNA ITSN1 expressions with disease risk, severity, and inflammatory cytokine levels in coronary artery disease (CAD) patients.
  • To determine the potential of these lncRNAs as biomarkers for CAD.

Main Methods:

  • A case-control study involving 191 participants (102 CAD patients, 89 controls) undergoing coronary angiography.
  • Quantitative polymerase chain reaction (qPCR) was used to measure plasma lncRNA IFNG-AS1, lncRNA ANRIL, and lncRNA ITSN1 expressions.
  • Serum levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8, IL-10, IL-17) were assessed using ELISA, and Gensini Score evaluated CAD severity.

Main Results:

  • Circulating lncRNA IFNG-AS1 was significantly upregulated in CAD patients compared to controls (P < .001).
  • lncRNA IFNG-AS1 expression showed a strong predictive value for CAD risk (AUC = 0.755) and was positively correlated with disease severity (Gensini Score, P = .009).
  • lncRNA IFNG-AS1 levels were positively associated with inflammatory markers (hs-CRP, TNF-α, IL-6) and negatively with IL-10, while lncRNA ANRIL and lncRNA ITSN1 showed no significant correlations.

Conclusions:

  • Circulating lncRNA IFNG-AS1 expression is a potential biomarker for increased risk and severity of coronary artery disease.
  • Elevated lncRNA IFNG-AS1 levels are associated with heightened systemic inflammation in CAD patients.
  • lncRNA ANRIL and lncRNA ITSN1 do not appear to be significantly associated with CAD risk or severity in this cohort.
Abstract

Keywords:
Gensini Scorecoronary artery diseasedisease riskinflammatory cytokineslncRNA IFNG-AS1

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