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Updated: Feb 10, 2026

Microplot Design and Plant and Soil Sample Preparation for 15Nitrogen Analysis
Published on: May 10, 2020
[Effects of grafting and nitrogen fertilization on melon yield and nitrogen uptake and utilization]
Liang Xue1, Zhong Ming Ma2, Shao Ping DU3
1Institute of Soil, Fertilizer and Water-saving Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.
Abstract:
A split-field design experiment was carried out using two main methods of cultivation (grafting and self-rooted cultivation) and subplots with different nitrogen application levels (0, 120, 240, and 360 kg N·hm-2) to investigate the effects of cultivation method and nitrogen application levels on the yield and quality of melons, nitrogen transfer, nitrogen distribution, and nitrogen utilization rate. The results showed that melons produced by grafting cultivation had a 7.3% increase in yield and a 0.16%-3.28% decrease in soluble solid content, compared to those produced by self-rooted cultivation. The amount of nitrogen accumulated in melons grafted in the early growth phase was lower than that in self-rooted melons, and higher after fruiting. During harvest, nitrogen accumulation amount in grafted melon plants was 5.2% higher than that in self-rooted plants and nitrogen accumulation amount in fruits was 10.3% higher. Grafting cultivation increased the amount of nitrogen transfer from plants to fruits by 20.9% compared to self-rooted cultivation. Nitrogen distribution in fruits was >80% in grafted melons, whereas that in self-rooted melons was <80%. Under the same level of nitrogen fertilization, melons cultivated by grafting showed 1.3%-4.2% increase in nitrogen absorption and utilization rate, 2.73-5.56 kg·kg-1 increase in nitrogen agronomic efficiency, and 7.39-16.18 kg·kg-1 increase in nitrogen physiological efficiency, compared to self-rooted cultivation. On the basis of the combined perspective of commercial melon yield, and nitrogen absorption and utilization rate, an applied nitrogen amount of 240 kg·hm-2 is most suitable for graf-ting cultivation in this region.
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