[Rapid screening and construction of high-efficient petroleum-degrading microflora through enzyme activity assay]

It is of great practical significance to rapidly screen the mixed microbial flora for degradation of complex organic pollutants. It was the first time to rapidly calibrate the petroleum hydrocarbon degradation ability of highly efficient degrading microflora by measuring enzyme activity analyzed by the MicroResp^TM technology. The results were validated by the traditional shaking culture. According to the activity analysis of exoenzymes, endoenzymes and mixed enzymes, the degradation abilities of microflora and enzymes (including exoenzymes, endoenzymes and mixed enzymes) on petroleum hydrocarbon was investigated. The results showed that the enzyme activity assay had perfect sensitivity and high-throughput for rapidly detecting the degradation ability of the petroleum hydrocarbon. The test period was 12 h which was 10 times shorter than shaking culture for 120 microflora combinations. The maximum degradation extent of the petroleum hydrocarbon by microflora reached (56.1±1.6)%. It was concluded that the method of enzyme activity assay was high-efficient and high-throughput screening, which could be used in the construction of petroleum-degrading microflora.