Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Glucose Absorption Into the Small Intestine

Glucose Absorption Into the Small Intestine

Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...

Methods for Studying Drug Absorption: In vitro

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

Anatomy of the Intestines

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...

Drug Absorption: Factors Affecting GI Absorption

Drug Absorption: Factors Affecting GI Absorption

The process of oral drug absorption can be influenced by several factors. Weakly acidic drugs tend to be absorbed more readily from the stomach due to their nonionized state. However, absorption may be less efficient in the upper intestine, where drugs are often ionized. Interestingly, despite the stomach's apparent advantage for drug absorption, its mucous layer can hinder diffusion. Its surface area is also smaller than the intestine's, which can further slow down the absorption rate.

Equivalence: In Vitro and In Vivo Bioequivalence

Equivalence: In Vitro and In Vivo Bioequivalence

Body:Bioequivalence studies are crucial in evaluating whether new drugs can match an approved one regarding pharmacological effects and clinical performance. These studies test if drugs, despite different dosage forms, share identical plasma concentration-time profiles. Three types of equivalence are central to these studies: chemical, pharmaceutical, and therapeutic. Chemical equivalence indicates that two or more drug products contain identical active ingredients in equal amounts.

Absorption of Radiation

Absorption of Radiation

The rate of heat transfer by emitted radiation is described by the Stefan-Boltzmann law of radiation:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Generation and characterization of the hiPSC line CSSi023-A (16154) from a patient with ADOA caused by an OPA1 variant.

Stem cell research·2026

Same author

Effects of Enniatin B1 on differentiated Caco-2 human intestinal cells in vitro.

Mycotoxin research·2026

Same author

The "Bergamo Approach" for Pediatric and Adolescent Polytrauma-A One-Center Experience.

Children (Basel, Switzerland)·2025

Same author

Generation and characterization of a patient-derived iPSC line, CSSi022-A (15666), with a pathogenic MFN2 mutation causing Charcot-Marie-Tooth disease type 2A.

Stem cell research·2025

Same author

Recent therapeutic advances in the treatment and management of amyotrophic lateral sclerosis: the era of regenerative medicine.

Expert review of neurotherapeutics·2025

Same author

Traffic-related diesel pollution particles impair the lysosomal functions of human iPSC-derived microglia.

Environment international·2025

Search research articles

Related Experiment Video

Updated: Feb 10, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Alpha-lactalbumin Effect on Myo-inositol Intestinal Absorption: In vivo and In vitro.

Giovanni Monastra¹, Yula Sambuy², Simonetta Ferruzza²

¹Department of Experimental Medicine, University of Rome "La Sapienza", Rome, Italy.

Current Drug Delivery

|May 11, 2018

Summary

This summary is machine-generated.

Oral administration of myo-inositol with α-lactalbumin enhances its absorption in healthy subjects. This combination may improve myo-inositol bioavailability, benefiting patients with impaired absorption.

Keywords:

Myo-inositol absorption enhancers bioactive peptides bioavailability healthy volunteers intestinal permeability pharmacokinetics α-lactalbumin.

More Related Videos

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

Related Experiment Videos

Last Updated: Feb 10, 2026

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Recapitulating Suckling-to-Weaning Transition In Vitro using Fetal Intestinal Organoids

Published on: November 15, 2019

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

Area of Science:

Nutritional Biochemistry
Gastroenterology
Pharmacokinetics

Background:

Myo-inositol is a vital molecule with therapeutic uses, but poor oral absorption can limit its effectiveness.
Understanding factors that enhance myo-inositol absorption is crucial for optimizing clinical outcomes.

Purpose of the Study:

To investigate if co-administering α-lactalbumin with myo-inositol improves myo-inositol plasma levels in healthy individuals.
To explore the in vitro mechanisms underlying myo-inositol absorption enhancement by α-lactalbumin.

Main Methods:

A two-phase in vivo study in healthy volunteers involving single oral doses of myo-inositol alone and with α-lactalbumin.
Plasma myo-inositol concentrations (Cmax, Tmax, AUC) were measured over time.
In vitro studies using Caco-2 cells assessed myo-inositol passage with and without digested α-lactalbumin, monitoring tight junction permeability.

Main Results:

Concomitant administration of α-lactalbumin significantly increased plasma myo-inositol concentrations compared to myo-inositol alone.
While Tmax was similar, the overall bioavailability of myo-inositol was enhanced.
In vitro, digested α-lactalbumin improved myo-inositol absorption in Caco-2 cells, correlating with increased tight junction permeability.

Conclusions:

Oral co-administration of myo-inositol with α-lactalbumin improves its absorption, potentially benefiting non-responder patients.
Preliminary evidence suggests α-lactalbumin-derived peptides may enhance myo-inositol absorption by modulating intestinal tight junction permeability.