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Updated: Feb 10, 2026

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Immunoelectron microscopic localization of Collagen type XV during human mesenchymal stem cells mineralization
Cristina Manferdini1, Nicoletta Zini2,3, Elena Gabusi1
1a SC Laboratory of Immunorheumatology and Tissue Regeneration , Rizzoli Orthopaedic Institute , Bologna , Italy.
Abstract:
Purpose/Aim of the study. Collagen type XV (ColXV) was identified, in our previews studies, as a novel component of bone extracellular matrix. The present study aims to investigate ColXV localization during mineralization of osteodifferentiated human mesenchymal stem cells (hMSCs).
Material And Methods:
hMSCs cultured in osteogenic medium have been analyzed at day 14 and 28 for mineral matrix deposition by alizarin red S staining, ultrastructural analysis and ColXV localization by immunogold electron microscopy.
Results:
Our data show an intimate association between ColXV and fibrillar components in areas localized far from mineralized nodules.
Conclusions:
We have demonstrated the efficacy of ultrastructural analysis, combined with immunocytochemistry, to establish a temporal and spatial localization of ColXV. This data, added to previous evidences, contribute to validate the negative effects of calcium deposits on ColXV expression.
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