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Related Experiment Video

Updated: Feb 10, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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System-based strategies for p53 recovery.

Muhammad Rizwan Azam1, Sahar Fazal2, Mukhtar Ullah3

  • 1CASPR, Department of Electronics Engineering, Capital University of Science and Technology, Islamabad, Pakistan.

IET Systems Biology
|May 11, 2018
PubMed
Summary

This study introduces a systems theory approach to design drugs targeting the p53 pathway. A novel control strategy reactivates wild-type p53, offering potential for cancer therapy.

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Area of Science:

  • Systems biology
  • Control engineering
  • Mathematical modeling

Background:

  • The p53 pathway is crucial in cancer, often dysregulated.
  • Reactivating wild-type p53 is a therapeutic goal.
  • Existing models require enhancement to include drug effects.

Purpose of the Study:

  • To develop a systems theory-based drug design for the p53 pathway.
  • To re-activate wild-type p53 using control engineering principles.
  • To incorporate drug pharmacokinetics into the model.

Main Methods:

  • Mathematical modeling using ordinary differential equations.
  • Control system analysis and controller design.
  • Attractor point and bifurcation analysis.
  • Incorporation of Nutlin effects and pharmacokinetic modeling.

Main Results:

  • A modified mathematical model of the p53 pathway including Nutlin.
  • A two-loop negative feedback control strategy for p53 re-activation.
  • Analysis of sustained and oscillatory p53 revival.
  • Framework for integrating pharmacokinetic effects.

Conclusions:

  • Systems theory and control engineering offer a novel approach to drug design for the p53 pathway.
  • The proposed control strategy can effectively re-activate wild-type p53.
  • The integrated framework provides a basis for optimizing drug interventions in cancer therapy.