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Updated: Feb 10, 2026

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device
Published on: March 22, 2022
Improving siRNA Delivery In Vivo Through Lipid Conjugation
Maire F Osborn1, Anastasia Khvorova1,2
11 RNA Therapeutics Institute, University of Massachusetts Medical School , Worcester, Massachusetts.
Abstract:
RNA interference (RNAi)-based therapeutics are approaching clinical approval for genetically defined diseases. Current clinical success is a result of significant innovations in the development of chemical architectures that support sustained, multi-month efficacy in vivo following a single administration. Conjugate-mediated delivery has established itself as the most promising platform for safe and targeted small interfering RNA (siRNA) delivery. Lipophilic conjugates represent a major class of modifications that improve siRNA pharmacokinetics and enable efficacy in a broad range of tissues. Here, we review current literature and define key features and limitations of this approach for in vivo modulation of gene expression.
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