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Decreasing Function01:27

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A decreasing function describes a relationship where the output consistently declines as the input increases. This means that for any two input values, if one is greater than the other, the corresponding output is smaller. Mathematically, a function f is decreasing on an interval I if for every x1 < x2​ in I, f (x1) > f (x2). This type of behavior is visually identified on a graph that slopes downward from left to right.The nature of a function can be analyzed by calculating...
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Updated: Feb 10, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
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A novel functional polymorphism of GFAP decrease glioblastoma susceptibility through inhibiting the binding of

Jie Wang1, Ming-Lei Wang2, Chang-Hui Wang1

  • 1Department of Neurosurgery, The Shanghai Neuromedical Center, Qingdao University, Shanghai, China.

Aging
|May 11, 2018
PubMed
Summary

Genetic variations in the Glial fibrillary acidic protein (GFAP) gene, specifically SNP rs11558961, impact glioblastoma (GBM) chemoresistance and metastasis. This SNP influences miR-139 binding, affecting GBM cell progression and susceptibility.

Keywords:
GFAPchemoresistancefunctional polymorphismglioblastomametastasismiR-139

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Area of Science:

  • Neuro-oncology
  • Molecular Genetics
  • Cancer Biology

Background:

  • Glioblastoma (GBM) is a prevalent CNS tumor with unclear genetic underpinnings.
  • Glial fibrillary acidic protein (GFAP) is implicated in GBM aggressiveness and poor survival.
  • Understanding genetic factors is crucial for improving GBM treatment strategies.

Purpose of the Study:

  • To investigate the association between GFAP single nucleotide polymorphisms (SNPs) and GBM chemoresistance and metastasis.
  • To elucidate the molecular mechanisms by which GFAP SNPs influence GBM progression.
  • To identify potential genetic markers for optimizing GBM clinical trials and personalized therapies.

Main Methods:

  • Genotyping of four tagging SNPs in GFAP within a cohort of 667 subjects.
  • Bioinformatic analysis of significant SNPs using online tools.
  • Functional experiments assessing SNP effects on gene expression, microRNA binding, chemoresistance, and cell migration.

Main Results:

  • SNP rs11558961 was significantly associated with GBM susceptibility.
  • The rs11558961 variant was predicted to affect miR-139 binding to the GFAP 3'UTR.
  • The G-allele of rs11558961 reduced GBM cell chemoresistance and migration, correlating with increased miR-139 responsiveness.

Conclusions:

  • The GFAP SNP rs11558961 influences GBM chemoresistance and progression by modulating miR-139 binding.
  • This genetic variant may decrease GBM susceptibility through its effects on miR-139.
  • Findings offer insights for refining clinical trial designs and personalizing GBM treatment approaches.