Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubules01:35

Microtubules

100.6K
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
100.6K
Microtubules01:18

Microtubules

10.8K
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
10.8K
Genetic Screens02:46

Genetic Screens

5.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
5.8K
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

6.0K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
6.0K
Microtubule Associated Motor Proteins01:32

Microtubule Associated Motor Proteins

10.8K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
10.8K
Actin Filament Depolymerization01:19

Actin Filament Depolymerization

4.0K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evidence for early evolution of sulfated peptide signaling in plant development.

Plant physiology·2026
Same author

Oxygen‑sensing histone demethylase KDM6A modulates chondrocyte‑to‑osteoblast transdifferentiation by activating the Wnt/β‑catenin pathway.

International journal of molecular medicine·2026
Same author

Clinical Validation of the Belay Ascentâ„¢ Test to Report on Chromosomal Arm-Level Aneuploidy and Gene-Level Copy Number Variants in Cerebrospinal Fluid Using Low-Pass Whole-Genome Sequencing.

Cancers·2026
Same author

Septin-mediated coupling of protein import and division during chloroplast evolution.

bioRxiv : the preprint server for biology·2026
Same author

Influence of computed tomography reconstruction algorithms on coronary artery calcium scores and reader agreement.

Journal of cardiovascular computed tomography·2026
Same author

Role of P450 enzymes of the Cyp2abfgs gene subfamilies in tobacco smoke-induced lung tumorigenesis in mice.

Carcinogenesis·2026

Related Experiment Video

Updated: Feb 10, 2026

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

1.9K

Conditional genetic screen in Physcomitrella patens reveals a novel microtubule depolymerizing-end-tracking protein.

Xinxin Ding1,2, Leah M Pervere1,2, Carl Bascom3,4

  • 1Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA.

Plos Genetics
|May 11, 2018
PubMed
Summary

Researchers identified a novel gene, CLoG1, essential for plant cell growth using the moss Physcomitrella patens. This discovery aids in understanding fundamental growth mechanisms by studying conditional mutants with impaired growth.

More Related Videos

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.9K
Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
04:54

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

41.6K

Related Experiment Videos

Last Updated: Feb 10, 2026

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

1.9K
Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

14.9K
Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens
04:54

Efficient Polyethylene Glycol PEG Mediated Transformation of the Moss Physcomitrella patens

Published on: April 19, 2011

41.6K

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Cell Biology

Background:

  • Identifying essential genes for cell growth and division is challenging due to lethality upon loss.
  • Conditional mutants offer a strategy to study gene function by observing phenotypes under specific conditions.

Purpose of the Study:

  • To identify novel genes regulating plant cell growth using conditional loss-of-growth (CLoG) mutants in Physcomitrella patens.
  • To characterize the function and localization of the newly identified CLoG1 gene.

Main Methods:

  • Generation and screening of conditional mutants in Physcomitrella patens at high temperatures.
  • Whole-genome sequencing of pooled segregants to identify mutations.
  • Phenotypic analysis and fluorescent protein tagging for gene localization and function studies.

Main Results:

  • A novel gene, CLoG1, was identified in conditional mutants exhibiting impaired cell growth at restrictive temperatures.
  • CLoG1 is crucial for cell growth but not essential for cell division, with smaller cells observed in clog1 mutants.
  • CLoG1 localizes to microtubules and regulates microtubule dynamics, impacting plant growth.

Conclusions:

  • Physcomitrella patens is a valuable model for studying essential plant cell growth genes.
  • The CLoG1 gene plays a critical role in regulating microtubule dynamics and promoting plant cell growth.
  • This study expands our understanding of the genetic control of plant cell growth and provides a new tool for future research.