microRNA-375 modulates the NF-κB pathway in miiuy croaker by targeting DUSP1 gene

Area of Science:

• Molecular Biology
• Genomics
• Fish Biology

Background:

• microRNAs (miRNAs) are small non-coding RNAs regulating biological processes.
• Dual-Specificity Phosphatases (DUSPs), particularly DUSP1, are key in MAPK regulation.
• DUSP1's role in fish gene regulation remains understudied.

Purpose of the Study:

• To investigate the regulatory role of miRNAs on DUSP1 in fish.
• To elucidate the specific interaction between miR-375 and DUSP1.
• To understand the impact of this regulation on cellular signaling pathways.

Main Methods:

• Dual-luciferase reporter assay to confirm miRNA-target interaction.
• Western blotting to assess protein level changes.
• Utilized miR-375 mimics and pre-miR-375 plasmid in miiuy croaker.

Main Results:

• miR-375 was confirmed to negatively regulate DUSP1 expression in miiuy croaker.
• The interaction occurs via binding to the 3' untranslated region of the DUSP1 gene.
• miR-375 negatively impacts the NF-κB signaling pathway by targeting DUSP1.

Conclusions:

• miR-375 acts as a negative regulator of DUSP1 in teleost fish.
• This interaction provides insights into miRNA-mediated gene expression control in fish.
• The findings contribute to understanding complex genomic regulatory networks in fish.