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Updated: Feb 10, 2026

Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay
Published on: October 30, 2011
An anatomic transcriptional atlas of human glioblastoma
Ralph B Puchalski1,2, Nameeta Shah3,4, Jeremy Miller5
1Allen Institute for Brain Science, Seattle, WA 98109, USA. mikeh@alleninstitute.org rbpuchalski@gmail.com nameeta.shah@gmail.com.
Abstract:
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, and their relationships to histologic features routinely used for diagnosis are unclear. We present the Ivy Glioblastoma Atlas, an anatomically based transcriptional atlas of human glioblastoma that aligns individual histologic features with genomic alterations and gene expression patterns, thus assigning molecular information to the most important morphologic hallmarks of the tumor. The atlas and its clinical and genomic database are freely accessible online data resources that will serve as a valuable platform for future investigations of glioblastoma pathogenesis, diagnosis, and treatment.
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