High-Speed Lateral Flow Strategy for a Fast Biosensing with an Improved Selectivity and Binding Affinity.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Conventional biosensors often face limitations in reaction speed, selectivity, and performance under harsh environmental conditions.
• Improving the efficiency of molecular interactions on biosensor surfaces is crucial for advancing diagnostic capabilities.

Purpose of the Study:

• To develop a high-speed lateral flow strategy that enhances biosensing performance, including selectivity and binding affinity.
• To demonstrate the effectiveness of this strategy under various challenging conditions, such as low pH and low ionic strength.

Main Methods:

• A novel biosensing strategy involving a rotating disk to generate high-speed lateral flow of the target solution over immobilized biosensors.
• Experimental validation of the strategy using target molecules and sensing molecules under controlled laboratory conditions.

Main Results:

• Achieved significantly high reaction speeds and binding affinities for target molecules.
• Demonstrated low nonspecific adsorption of target molecules to biosensors.
• Observed enhanced binding affinity even under harsh conditions like low pH and low ionic strength.

Conclusions:

• The developed high-speed lateral flow strategy effectively improves the performance of conventional biosensors.
• This approach offers a simple yet powerful tool for versatile bio and medical applications, enhancing diagnostic speed and reliability.