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Updated: Feb 10, 2026

Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
The effect of storage temperature on the biological activity of extracellular vesicles for the complement system
Sang June Park1, Hyungtaek Jeon1, Seung-Min Yoo1
1Department of Microbiology and Immunology, Eulji University School of Medicine, 77 Gyeryong-ro 771 beon-gil, Jung-gu, Daejeon, 34824, South Korea.
Abstract:
Extracellular vesicles (EVs) are mediators of intercellular communication by transporting cargo containing proteins, lipids, mRNA, and miRNA. There is increasing evidence that EVs have various roles in regulating migration, invasion, stemness, survival, and immune functions. Previously, we have found that EVs from Kaposi's sarcoma-associated herpesvirus (KSHV)-infected human endothelial cells have the potential to activate the complement system. Although many studies have shown that the physical properties of EVs can be changed by their storage condition, there have been few studies for the stability of biological activity of EVs in various storage conditions. In this study, we investigated various conditions to identify the best conditions to store EVs with functional stability for 25 d. Furthermore, the correlation between the function and other characteristics of EVs, including the expression of EV markers, size distribution, and particle number, were also analyzed. Our results demonstrated that storage temperature is an important factor to maintain the activity of EVs and would be useful information for basic research and clinical application using EVs.
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