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Updated: Feb 10, 2026

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Published on: February 9, 2018
Preparative isolation of ganoderic acid S, ganoderic acid T and ganoderol B from Ganoderma lucidum mycelia by
Na Feng1, Yutian Wei2, Jie Feng1
1Ministry of Agriculture, People's Republic of China, National Engineering Research Center of Edible Fungi and National R&D Center for Edible Fungi Processing, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences; Key Laboratory of Edible Fungi Resources and Utilization (South), Shanghai, China.
Abstract:
Ganoderic acid S, ganoderic acid T and ganoderal B are the main bioactive triterpenes of Ganoderma lucidum. In this study, mycelia of G. lucidum were obtained by two-stage fermentation and then extracted by ethanol and petroleum ether sequentially to obtain crude triterpenes. The crude sample was further purified by recycling high-speed counter-current chromatography with n-hexane-ethyl acetate-methanol-water (7:12:11:5, v/v/v/v) as the optimized two-phase solvent system. A 16.4 mg aliquot of ganoderol B with a purity of 90.4% was separated from 300 mg of the crude sample in a single run. After employing the recycling elution mode of HSCCC with n-hexane-ethyl acetate-methanol-water (6:10:8:4.5, v/v/v/v) for five cycles, 25.7 mg ganoderic acid T and 3.7 mg ganoderic acid S with purities of 97.8 and 83.0%, respectively, were obtained. The purities of three compounds were determined by high-performance liquid chromatography and their chemical structures were identified by NMR and MS data.
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