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LSD1 negatively regulates autophagy through the mTOR signaling pathway in ovarian cancer cells

Ye Wei1, Tiantian Han1, Ranran Wang1

  • 1School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China.

Oncology Reports
|May 12, 2018
PubMed

Insights

Lysine-specific demethylase 1 (LSD1) suppresses autophagy in ovarian cancer cells. Inhibiting LSD1 boosts autophagy, suggesting it

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • Lysine-specific demethylase 1 (LSD1) is implicated in cell proliferation, differentiation, and cancer.
  • Autophagy is a cellular degradation process involved in various diseases, including cancer.

Purpose of the Study:

  • To investigate the role of LSD1 in regulating autophagy in ovarian cancer.
  • To elucidate the molecular mechanisms by which LSD1 affects autophagy in ovarian cancer cells.

Main Methods:

  • Ovarian cancer HO8910 cells were used.
  • Pharmacological inhibition and genetic knockdown of LSD1 were performed.
  • Autophagy markers (LC3-II), autophagosome formation, and autophagic flux were analyzed.
  • The mTOR signaling pathway was investigated.

Main Results:

  • LSD1 was identified as an autophagy suppressor in ovarian cancer cells.
  • Inhibition or knockdown of LSD1 increased LC3-II levels, autophagosome formation, and autophagic flux.
  • LSD1 knockdown enhanced autophagy induced by serum starvation and rapamycin.
  • LSD1 was found to regulate autophagy via the mTOR signaling pathway.

Conclusions:

  • LSD1 is a novel negative regulator of autophagy in ovarian cancer via the mTOR pathway.
  • LSD1 may drive ovarian cancer progression by disrupting autophagy.

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