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miR‑188 suppresses tumor progression by targeting SOX4 in pediatric osteosarcoma

Lu Pan1, Lingxin Meng2, Feng Liang1

  • 1Department of Pediatric Surgery, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China.

Molecular Medicine Reports
|May 12, 2018
PubMed

Insights

MicroRNA-188 (miR-188) is downregulated in pediatric osteosarcoma (OS). Restoring miR-188 suppresses OS cell growth and invasion by targeting SOX4, indicating its tumor-suppressive role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-188 (miR-188) is a known tumor suppressor in various cancers.
  • Its role in pediatric osteosarcoma (OS) remains unexplored.

Purpose of the Study:

  • To investigate the function and mechanism of miR-188 in pediatric osteosarcoma.
  • To determine if miR-188 acts as a tumor suppressor in OS.

Main Methods:

  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to assess miR-188 expression.
  • In vitro assays (proliferation, migration, invasion, apoptosis) to evaluate miR-188 function.
  • In vivo assays to assess tumor growth inhibition.
  • Target validation by assessing SOX4 regulation and restoration experiments.

Main Results:

  • miR-188 expression was significantly downregulated in pediatric OS tissues and cell lines.
  • miR-188 overexpression suppressed OS cell proliferation, migration, and invasion, and induced apoptosis.
  • In vivo studies showed miR-188 overexpression inhibited tumor growth.
  • miR-188 directly targeted SOX4, and its expression was inversely correlated with SOX4 in OS tissues.
  • Restoration of SOX4 reversed the inhibitory effects of miR-188.

Conclusions:

  • miR-188 functions as a tumor suppressor in pediatric osteosarcoma.
  • miR-188 suppresses OS progression by targeting and downregulating SOX4.

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