Related Experiment Videos
Translational signals of a major head protein gene of bacteriophage lambda
1Department of Molecular Genetics, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Abstract:
The D gene of bacteriophage lambda which codes for a major head protein is expressed at a high level during lytic growth. We have constructed a set of D-lacZ gene fusions in order to examine the factors determining the high efficiency of the D translational initiation signals. It was found that an integral sequence, 300 bp long and upstream of the ATG initiation codon, is required for maximal protein synthesis.