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08:53
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Published on: May 7, 2018
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Sphingomyelin ability to act as chiral selector using nanodisc electrokinetic chromatography
William M Penny1, Christopher P Palmer1
1Department of Chemistry and Biochemistry, University of Montana, Missoula, MT 59803, United States.
Chemistry and Physics of Lipids
|May 14, 2018
Summary
This study demonstrates stereoselective interactions between chiral molecules and cell membranes using nanodisc electrokinetic chromatography. This finding advances our understanding of drug development and membrane interactions.
Area of Science:
- Biochemistry
- Pharmacology
- Analytical Chemistry
Background:
- Stereochemistry significantly impacts drug distribution and metabolism.
- Cell membrane interactions and passive diffusion were previously thought to be non-stereoselective.
- Conflicting research exists on the stereoselectivity of lipid bilayer interactions.
Purpose of the Study:
- To investigate stereoselective interactions between chiral molecules and lipid bilayers.
- To demonstrate the utility of nanodisc electrokinetic chromatography in detecting subtle affinity differences.
- To explore stereoselectivity in drug-membrane interactions.
Main Methods:
- Utilized nanodisc electrokinetic chromatography.
- Employed a pair of atropisomers, R-(+)/(S)-(-) 1,1'-Bi-2-naphthol, as chiral probes.
- Tested various enantiomer and diastereomer probes with sphingomyelin nanodisc bilayers.
Main Results:
- Demonstrated stereoselective interactions between R-(+)/(S)-(-) 1,1'-Bi-2-naphthol and sphingomyelin nanodisc bilayers.
- Nanodisc electrokinetic chromatography proved sensitive to minor affinity variations.
- No stereoselectivity was observed with other tested enantiomer or diastereomer probes.
Conclusions:
- Stereoselective interactions with lipid bilayers are possible and can be detected.
- Nanodisc electrokinetic chromatography is a valuable tool for studying chiral molecule-membrane interactions.
- Further research is needed to understand the scope and implications of stereoselectivity in membrane transport.
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