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Capillary Electrophoresis: Instrumentation01:20

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Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Affinity capillary electrophoresis for studying interactions in life sciences.

Mais Olabi1, Matthias Stein1, Hermann Wätzig1

  • 1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Beethovenstr. 55, 38106 Braunschweig, Germany.

Methods (San Diego, Calif.)
|May 14, 2018
PubMed
Summary

Affinity capillary electrophoresis (ACE) analyzes biomolecular interactions using electrophoretic mobility shifts. Recent advancements have broadened its applications in free solution and immobilized techniques, enhancing preclinical study validation.

Keywords:
Affinity capillary electrophoresisFrontal analysis affinity capillary electrophoresisImmunoaffinity capillary electrophoresisMobility shift affinity capillary electrophoresisNon-equilibrium capillary electrophoresis of equilibrium mixturesProtein interactions

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Area of Science:

  • Analytical Chemistry
  • Biochemistry

Background:

  • Affinity capillary electrophoresis (ACE) is a technique that analyzes noncovalent interactions between ligands and analytes.
  • It is widely used for biomolecules like proteins, polysaccharides, and hormones.
  • ACE is valuable for validating high-throughput screening results in realistic media, such as body fluids.

Purpose of the Study:

  • To review literature on affinity capillary electrophoresis from 2016 to early 2018.
  • To highlight advancements in data processing and calculations for ACE techniques.
  • To showcase the expanded range of applications for both free solution and immobilized ACE.

Main Methods:

  • Literature review of Scopus and SciFinder databases.
  • Inclusion of keywords: "affinity capillary electrophoresis", "immunoaffinity capillary electrophoresis", "immunoassay capillary electrophoresis", and "immunosorbent capillary electrophoresis".
  • Selection and discussion of 112 articles from over 200 found.

Main Results:

  • Substantial improvements in data processing and calculations for mobility shift ACE (ms ACE), frontal analysis ACE (FA ACE), and plug-plug kinetic capillary electrophoresis (ppKCE).
  • Considerable broadening of applications for diverse free solution and immobilized ACE techniques.
  • ACE is recommended for animal testing and preclinical studies.

Conclusions:

  • Affinity capillary electrophoresis is a powerful analytical tool with expanding applications.
  • Advancements in ACE techniques enhance its predictive capabilities and utility in various research areas.
  • ACE is crucial for validating screening results and preparing for preclinical studies.