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Updated: Feb 10, 2026

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure
Published on: September 4, 2010
Thread spray mass spectrometry for direct analysis of capsaicinoids in pepper products
Sierra Jackson1, Devin J Swiner1, Patricia C Capone1
1Department of Chemistry and Biochemistry, The Ohio State University, 43210, Columbus, OH, United States.
Abstract:
Thread spray ambient ionization uses single threads as a medium for sampling and ionization. This approach was demonstrated through the detection of various capsaicinoids from the interior of pepper fruits without destruction of the sample. Pepper residues present on the thread were analyzed by the application of DC voltage and solvent to cause field-induced charged droplet generation. Capsaicinoids extracted from the sample are contained in the electrosprayed droplets and transported to the mass spectrometer for characterization. The thread spray mass spectrometry method was optimized using commercially available materials like 100% cotton, cotton:polyester (35/65), 100% polyester, and nylon fabrics and subsequently applied for in-situ analysis of six different pepper fruits and pepper spray residues on fabrics. The results indicated that the special physico-chemical characteristics of threads allowed a rapid and convenient sampling and ionization of pepper products for analysis by mass spectrometry. The total capsaicinoid ion yields for the various pepper products correlated very well with that reported in Scoville Heat Units, suggesting that quantitative assessment of pungency levels may be achieved via the direct sample analysis without prior separation.
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