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Updated: Feb 10, 2026

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
Published on: August 22, 2018
Design verification of a compact system for detecting tissue perfusion using bimodal diffuse optical technologies
Julia M Pakela1, Taylor L Hedrick1, Seung Yup Lee1
1University of Michigan (United States).
Abstract:
It is essential to monitor tissue perfusion during and after reconstructive surgery, as restricted blood flow can result in graft failures. Current clinical procedures are insufficient to monitor tissue perfusion, as they are intermittent and often subjective. To address this unmet clinical need, a compact, low-cost, multimodal diffuse correlation spectroscopy and diffuse reflectance spectroscopy system was developed. We verified system performance via tissue phantoms and experimental protocols for rigorous bench testing. Quantitative data analysis methods were employed and tested to enable the extraction of tissue perfusion parameters. This design verification study assures data integrity in future in vivo studies.
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