Glucose Acutely Reduces Cytosolic and Mitochondrial H₂O₂ in Rat Pancreatic Beta Cells

Whether H₂O₂ contributes to the glucose-dependent stimulation of insulin secretion (GSIS) by pancreatic β cells is highly controversial. We used two H₂O₂-sensitive probes, roGFP2-Orp1 (reduction/oxidation-sensitive enhanced green fluorescent protein fused to oxidant receptor peroxidase 1) and HyPer (hydrogen peroxide sensor) with its pH-control SypHer, to test the acute effects of glucose, monomethyl succinate, leucine with glutamine, and α-ketoisocaproate on β cell cytosolic and mitochondrial H₂O₂ concentrations. We then tested the effects of low H₂O₂ and menadione concentrations on insulin secretion. RoGFP2-Orp1 was more sensitive than HyPer to H₂O₂ (response at 2-5 vs. 10 μM) and less pH-sensitive. Under control conditions, stimulation with glucose reduced mitochondrial roGFP2-Orp1 oxidation without affecting cytosolic roGFP2-Orp1 and HyPer fluorescence ratios, except for the pH-dependent effects on HyPer. However, stimulation with glucose decreased the oxidation of both cytosolic probes by 15 μM exogenous H₂O₂. The glucose effects were not affected by overexpression of catalase, mitochondrial catalase, or superoxide dismutase 1 and 2. They followed the increase in NAD(P)H autofluorescence, were maximal at 5 mM glucose in the cytosol and 10 mM glucose in the mitochondria, and were partly mimicked by the other nutrients. Exogenous H₂O₂ (1-15 μM) did not affect insulin secretion. By contrast, menadione (1-5 μM) did not increase basal insulin secretion but reduced the stimulation of insulin secretion by 20 mM glucose. Subcellular changes in β cell H₂O₂ levels are better monitored with roGFP2-Orp1 than HyPer/SypHer. Nutrients acutely lower mitochondrial H₂O₂ levels in β cells and promote degradation of exogenously supplied H₂O₂ in both cytosolic and mitochondrial compartments. The GSIS occurs independently of a detectable increase in β cell cytosolic or mitochondrial H₂O₂ levels.