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DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
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Related Experiment Video

Updated: Feb 10, 2026

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System.

Joseph C Siefert¹, Emily A Clowdus¹, Duane Goins²

¹Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation; Department of Cell Biology, University of Oklahoma Health Sciences Center.

Journal of Visualized Experiments : Jove

|May 15, 2018

Summary

Zebrafish protocols enable high-resolution DNA replication timing analysis. This research explores replication timing

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Last Updated: Feb 10, 2026

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

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Published on: October 27, 2011

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

Area of Science:

Genomics
Developmental Biology
Cancer Research

Background:

DNA replication timing is crucial for genome stability, influencing chromatin structure, transcription, and mutation rates.
Replication timing alterations are observed during development and in cancer, yet their functional roles remain unclear.
Zebrafish offer a powerful in vivo model for studying DNA replication timing dynamics.

Purpose of the Study:

To detail protocols for determining genome-wide DNA replication timing in zebrafish.
To enable investigation into the role of replication timing in development and disease.

Main Methods:

Cell sorting from zebrafish embryos and adults.
High-resolution, genome-wide DNA replication timing pattern construction.
Analysis of DNA copy number changes via next-generation sequencing data.

Main Results:

Established protocols for high-resolution DNA replication timing analysis in zebrafish.
Demonstrated the utility of the zebrafish model for in vivo replication timing studies.

Conclusions:

The developed zebrafish methods facilitate studies on replication timing establishment and maintenance during development.
This model system can be used to investigate replication timing's role in mutations, tumorigenesis, and disease.
Enables research into the effects of perturbing replication timing on biological processes.