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Visualizing Dengue Virus through Alexa Fluor Labeling
Published on: July 9, 2011
Identification of Compound-B, a novel anti-dengue virus agent targeting the non-structural protein 4A
Haruaki Nobori1, Shinsuke Toba1, Ryu Yoshida2
1Drug Discovery & Disease Research Laboratory, Shionogi & Co., Ltd., Osaka, Japan; Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Abstract:
Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic fever/dengue shock syndrome. At present, no antiviral drugs are available for treatment DENV infections. In this study, a screening system based on a DENV-infected cell-based assay identified a novel anti-DENV agent with a benzimidazole skeleton, named Compound-B, which demonstrated antiviral activity specific to four DENV serotypes (EC50: 1.32-4.12 μM). Analysis of a single amino acid substitution of Compound-B-resistant DENV2 revealed that mutation C87S in the non-structural protein 4A (NS4A) contributes to resistance to Compound-B.
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