Creation of deletion and insertion clonal complex 8 Staphylococcus aureus mutants using a common cloning vector
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Updated: Feb 10, 2026

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
Ping Chen1, S L Rajasekhar Karna1, Kai P Leung1
1Dental and Craniofacial Trauma Research and Tissue Regeneration Directorate, US Army Institute of Surgical Research, JBSA Fort Sam, Houston, TX 78234, United States.
Abstract:
Deletion and insertion clonal complex 8 Staphylococcus aureus mutants were created without using intermediate host S. aureus RN4220 or temperature-sensitive shuttle vectors. These mutants were created using a common cloning vector by passing the constructs through a modification host and recovering the electroporated cells in a large volume of medium.
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