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Updated: Feb 10, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
Highly sensitive detection of hepatitis C virus DNA by using a one-donor-four-acceptors FRET probe
Ping Zeng1, Peng Hou1, Chun Ju Jing1
1Key Laboratory on Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Abstract:
Sensitive detection of Hepatitis C virus (HCV) DNA is critical to treat and prevent the propagation of hepatitis C diseases. Herein a FRET probe of One-Donor-Multi-Acceptors (D-mA) assembly is designed through the formation of triple-helix DNA structure, which can greatly reduce the background of FRET system so as to greatly increase the detection sensitivity of hepatitis C virus DNA with the limit of detection (3σ) being 24.57 nmol/L, making the detection of clinic samples successful and showing that the newly developed FRET probe of D-mA assembly could be applied for the early diagnosis and treatment of hepatitis C disease.
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