A single nanoparticle-based real-time monitoring of biocatalytic progress and detection of hydrogen peroxide
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Updated: Feb 10, 2026

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Published on: October 20, 2023
Jie Pang1, Yun Zhao1, Hai-Ling Liu1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University, Nanjing 210023, PR China.
Abstract:
This paper reported a new method to observe the catalytic progress of the natural horseradish peroxidase (HRP) in-situ on single gold nanoparticles (GNPs) by the combination of dark field imaging and plasmonic resonance scattering spectra. The produced single HRP-GNP exhibited localized catalytic property toward H2O2-Diaminobenzidine (DAB), which could be used to detect the concentration of H2O2 in micro/nanospace. The linear range for H2O2 sensing was from 0.01 μM to 5 μM with a detection limit of 10 nM. The new design strategy could be applied for a broader bioanalysis situation by substituting the HRP with other specified biocatalyst.
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