Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on the murine interleukin-1 receptor.

T A Bird1, A J Gearing, J Saklatvala

  • 1Strangeways Research Laboratory, Cambridge, U.K.

Developments in Biological Standardization
|January 1, 1988
PubMed
Summary

Interleukin-1 (IL-1) binds to cell surface receptors, identified as 100 kDa and 80 kDa polypeptides. The smaller 80 kDa glycoprotein receptor interacts with both natural and recombinant IL-1 forms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Does decorin stabilize the extracellular matrix of articular cartilage and slow the progression of osteoarthritis?

Osteoarthritis and cartilage·2021
Same author

Transcriptional analysis of micro-dissected articular cartilage in post-traumatic murine osteoarthritis.

Osteoarthritis and cartilage·2014
Same author

Purification of murine monoclonal antibodies.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

FGF-2 is bound to perlecan in the pericellular matrix of articular cartilage, where it acts as a chondrocyte mechanotransducer.

Osteoarthritis and cartilage·2007
Same author

Basic fibroblast growth factor: an extracellular mechanotransducer in articular cartilage?

Biochemical Society transactions·2006
Same author

Development of a novel 2D proteomics approach for the identification of proteins secreted by primary chondrocytes after stimulation by IL-1 and oncostatin M.

Rheumatology (Oxford, England)·2006

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Interleukin-1 (IL-1) mediates diverse biological actions through specific cell surface receptors.
  • Understanding IL-1 receptor interactions is crucial for deciphering its signaling pathways.

Purpose of the Study:

  • To identify and characterize the cell surface receptors for Interleukin-1 (IL-1).
  • To investigate the binding properties of IL-1 to its receptors.

Main Methods:

  • Covalent crosslinking of natural porcine IL-1 and recombinant human IL-1 to murine EL-4 6.1 thymoma cells.
  • Solubilization and purification of functional IL-1 receptors.
  • Ligand-blotting assays to analyze receptor-ligand interactions.

Main Results:

  • Crosslinking identified two polypeptides (100 kDa and 80 kDa) involved in IL-1 recognition.
  • The 80 kDa polypeptide, a glycoprotein, demonstrated interaction with both natural and recombinant IL-1.
  • Soluble receptor studies confirmed the binding capabilities of the 80 kDa component.

Conclusions:

  • The 80 kDa glycoprotein is a key component of the IL-1 receptor complex.
  • This receptor subunit interacts with diverse forms of IL-1, suggesting a conserved binding mechanism.

Related Experiment Videos