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Updated: Feb 10, 2026

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
Published on: June 30, 2016
Culture of Human Monocyte-Derived Macrophages
Aoife Kelly1,2,3, Aleksander M Grabiec4, Mark A Travis5,6,7
1Manchester Collaborative Centre for Inflammation Research, University of Manchester, Manchester, UK. aoife.kelly@manchester.ac.uk.
Abstract:
The study of human macrophages is often hampered by access to tissue and inability of this cell type to survive in vitro following isolation. The culture of human monocyte-derived macrophages (MDMs) represents a tool to study macrophages, with monocytes known to give rise to tissue macrophages influenced by certain environmental cues. Here we describe a method of culturing monocyte-derived macrophages from CD14+ blood monocytes and polarization toward different macrophage phenotypes.
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