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Updated: Feb 10, 2026
07:06
Bone Marrow-derived Macrophage Production
Published on: November 22, 2013
75.4K
Growing Murine Bone Marrow-Derived Macrophages.
Anaïs Assouvie1,2,3,4, Lisa P Daley-Bauer5, Germain Rousselet6,7,8,9
1CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France.
Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2018
Summary
This chapter details the method for obtaining bone marrow-derived macrophages (BMDM) and preserving them through freezing. These versatile immune cells are crucial for various in vitro research applications.
Area of Science:
- Immunology
- Cell Biology
Background:
- Bone marrow-derived macrophages (BMDM) are essential primary immune cells for in vitro research.
- Macrophage colony-stimulating factor (M-CSF) is critical for their differentiation.
- BMDM offer advantages like high yield, storability, and derivation from genetically modified models.
Purpose of the Study:
- To present a standardized protocol for obtaining BMDM.
- To describe a reliable method for cryopreserving BMDM.
- To facilitate the use of BMDM in diverse experimental settings.
Main Methods:
- Isolation of bone marrow cells from mice.
- In vitro differentiation of bone marrow cells using M-CSF.
- Cryopreservation of differentiated BMDM.
Main Results:
- High yields of viable BMDM can be consistently obtained.
- Cryopreserved BMDM retain their functionality after thawing.
- The protocol is adaptable for various mouse strains.
Conclusions:
- The described method provides a robust approach for generating and storing BMDM.
- This facilitates reproducible in vitro immunological studies.
- The protocol supports the use of BMDM in research involving genetically modified mice.
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