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Updated: Feb 10, 2026

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages
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Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

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Isolation and Identification of Murine Serous Cavity Macrophages

Calum C Bain1, Stephen J Jenkins2

  • 1University of Edinburgh/MRC Centre for Inflammation Research, Queens Medical Research Institute, University of Edinburgh, Edinburgh, Scotland, UK. calum.bain@ed.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|May 16, 2018
PubMed

Insights

Peritoneal macrophages are key immune cells, but isolating them requires understanding complex cell populations. This study details methods to identify and isolate these critical cells for research.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • The peritoneal cavity is a common source for primary macrophages due to ease of access.
  • Multiparameter flow cytometry reveals complex mononuclear phagocyte populations in serous cavities.
  • Macrophages in serous cavities play roles in tissue homeostasis and inflammation.

Purpose of the Study:

  • To provide a comprehensive understanding of the peritoneal macrophage compartment.
  • To outline reliable strategies for identifying macrophages distinct from other mononuclear phagocytes.
  • To detail methods for isolating leukocytes from peritoneal and pleural cavities for ex vivo and in vitro analysis.

Main Methods:

  • Isolation of leukocytes from peritoneal and pleural cavities.
  • Application of multiparameter flow cytometry.
  • Development of strategies for identifying discrete mononuclear phagocyte populations.

Main Results:

  • Characterization of the complex cellular composition of serous cavity leukocyte populations.
  • Identification of distinct macrophage populations within the peritoneal cavity.
  • Establishment of reliable methods for distinguishing macrophages from dendritic cells and monocytes.

Conclusions:

  • Accurate identification and isolation of peritoneal macrophages are crucial for understanding their functions.
  • Detailed methodologies are presented to aid researchers in studying these cells.
  • This work facilitates deeper insights into tissue homeostasis and inflammatory processes involving macrophages.
Keywords:
Dendritic cellsIdentificationIsolationMacrophagesMonocytesPeritoneal cavityPleural cavity

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