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Related Experiment Video

Updated: Feb 10, 2026

A Macrophage-Tumor Spheroid Co-Invasion Assay

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

Phenotyping Tumor-Associated Macrophages.

R Ian Cumming¹, Yen-Rei A Yu²

¹Division of Pulmonary and Critical Care Medicine, Department of Medicine, Duke University School of Medicine, Durham, NC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 16, 2018

Summary

This study presents a flow cytometry method to analyze immune cells in tumor tissues. The approach enables detailed characterization of immune cell composition and function, including tumor-associated macrophages.

Keywords:

Dendritic cells Flow cytometry Granulocytes Immune cells Immunophenotyping Lymphocytes Macrophages Monocytes Mononuclear phagocytes Tumor-associated macrophages

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Area of Science:

Immunology
Cancer Biology
Cellular Biology

Background:

Tumor microenvironment research requires detailed immune cell analysis.
Current methods for immune cell characterization in nonlymphoid tissues can be complex.

Purpose of the Study:

To describe a standardized multi-parametric flow cytometry approach.
To enable comprehensive immune cell profiling in tumor-bearing murine nonlymphoid tissues.
To facilitate the phenotyping of tumor-associated macrophages.

Main Methods:

Processing of nonlymphoid tissues from tumor-bearing mice.
Utilizing a base antibody panel for broad immune cell identification.
Single staining condition for simultaneous analysis of multiple immune cell types.

Main Results:

Successful characterization of major immune cell types within nonlymphoid tissues.
Demonstration of the panel's utility for phenotyping tumor-associated macrophages.
Provides a foundation for examining immune cell composition, phenotype, and function.

Conclusions:

The described flow cytometry approach offers an efficient method for immune cell analysis in tumor tissues.
This technique aids in understanding the immune landscape of the tumor microenvironment.
Facilitates deeper investigation into the role of specific immune cells, such as macrophages, in cancer.