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Related Experiment Video

Updated: Feb 10, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking

A High-content Assay for Monitoring AMPA Receptor Trafficking

Published on: January 28, 2019

SNAP23-Kif5 complex controls mGlu1 receptor trafficking.

Fabrice Raynaud¹, Vincent Homburger¹, Martial Seveno²

¹IGF, CNRS, INSERM, Univ. Montpellier, Montpellier, France.

Journal of Molecular Cell Biology

|May 16, 2018

Summary

This summary is machine-generated.

The Kif5-SNAP23 complex is crucial for trafficking metabotropic glutamate receptor 1 (mGluR1) along dendrites. This interaction ensures proper mGluR1 cell surface expression in hippocampal neurons.

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Area of Science:

Neuroscience
Molecular Biology
Cell Biology

Background:

Metabotropic glutamate receptors (mGluRs) are vital for synaptic transmission in the mammalian brain.
Their roles in pathophysiology are significant, yet molecular mechanisms for intracellular transport remain unclear.

Purpose of the Study:

To identify molecular motors and adaptor proteins involved in trafficking and membrane localization of group I metabotropic glutamate mGlu1 receptors.
To elucidate the functional role of identified proteins in mGluR1 trafficking and cell surface expression.

Main Methods:

Proteomic analysis of cultured hippocampal neurons to identify interacting proteins with mGlu1 receptor.
Recombination techniques to modulate SNAP23-Kif5 interaction.
Fluorescence recovery after cleavage assays to assess receptor cell surface targeting.

Main Results:

Synaptosome-associated protein 23 (SNAP23) and kinesin motor Kif5 were identified as interacting proteins with mGlu1 receptor.
SNAP23 directly binds the carboxyl terminus of mGlu1 receptor.
The Kif5-SNAP23 complex regulates mGlu1 receptor trafficking along microtubules and its cell surface expression.

Conclusions:

The Kif5-SNAP23 complex is essential for the proper trafficking and cell surface expression of mGlu1 receptors in dendritic processes.
This study reveals key molecular determinants for postsynaptic receptor localization.