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CircRNA_0023642 promotes migration and invasion of gastric cancer cells by regulating EMT

L-H Zhou1, Y-C Yang, R-Y Zhang

  • 1Digestive Endoscopy Center, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chinese Academy of Sciences Sichuan Translational Medicine Research Hospital, Chengdu, Sichuan, China. llzmnq@126.com.

European Review for Medical and Pharmacological Sciences
|May 16, 2018
PubMed
Abstract

Insights

Circular RNAs (circRNAs) are dysregulated in cancer. This study reveals circRNA_0023642 promotes gastric cancer (GC) progression by enhancing cell migration and invasion, suggesting it as a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Non-coding circular RNAs (circRNAs) show altered expression in tumors.
  • The specific roles of circRNAs in cancer progression are not fully understood.
  • Gastric cancer (GC) remains a significant health concern with a need for novel therapeutic targets.

Purpose of the Study:

  • To investigate the expression, functions, and molecular mechanisms of circRNA_0023642 in gastric cancer.
  • To determine if circRNA_0023642 plays a role in GC progression.
  • To explore circRNA_0023642 as a potential therapeutic target for GC.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for expression analysis.
  • Cell proliferation, migration, invasion, and apoptosis assays (CCK-8, colony formation, transwell, flow cytometry).
  • Western blot to assess epithelial-mesenchymal transition (EMT) markers (N-cadherin, vimentin, snail, E-cadherin).

Main Results:

  • circRNA_0023642 was significantly upregulated in GC tissues and cell lines.
  • Downregulation of circRNA_0023642 inhibited GC cell proliferation, migration, and invasion, while inducing apoptosis.
  • circRNA_0023642 influences the EMT signaling pathway, impacting key regulatory proteins.

Conclusions:

  • circRNA_0023642 acts as a metastasis activator in GC by promoting EMT.
  • circRNA_0023642 represents a promising novel molecular therapeutic target for gastric cancer.

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