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Inhibition of thyroid carcinoma cells with YAP1 protein interference and its mechanism

C-Y Huang1, Z Han, X Li

  • 1Department of Breast and Thyroid Surgery, Hexian Memorial Hospital of Panyu District, Guangzhou, China. allenhz520@163.com.

European Review for Medical and Pharmacological Sciences
|May 16, 2018
PubMed
Abstract

Insights

Yes-associated protein 1 (YAP1) interference inhibits thyroid cancer cell growth. This mechanism involves the PI3K-Akt signaling pathway, offering potential therapeutic targets for thyroid carcinoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Thyroid carcinoma is a significant health concern.
  • The role of yes-associated protein 1 (YAP1) in thyroid cancer progression requires further elucidation.

Purpose of the Study:

  • To investigate the effects of YAP1 on thyroid carcinoma cells.
  • To elucidate the underlying mechanism of YAP1's action in thyroid cancer.

Main Methods:

  • Quantitative Real-time PCR (qRT-PCR) and Western blot were used to assess YAP1 expression in normal and thyroid cancer cell lines.
  • RNA interference (si-YAP1) was employed to study YAP1's functional impact.
  • Cell proliferation was evaluated using MTT assays, and the PI3K-Akt signaling pathway was analyzed via Western blot.

Main Results:

  • YAP1 expression was significantly elevated in thyroid carcinoma cells compared to normal cells, with the highest levels observed in the TPC-1 cell line.
  • Effective interference of YAP1 expression was confirmed by qRT-PCR and Western blot.
  • Suppression of YAP1 led to reduced cell proliferation and decreased expression of phosphorylated Akt (p-Akt).

Conclusions:

  • Interference with YAP1 effectively inhibits the growth of thyroid cancer cells.
  • The PI3K-Akt signaling pathway is implicated in the mechanism by which YAP1 influences thyroid cancer cell growth.

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