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[Experimental conditions of a technic of in vitro transcription using placental nuclei]
K Hess1, H el Ouardirhi, F Belleville
1Laboratoire de Biochimie Médicale I, Faculté de Médecine, Vandaeuvre-les-Nancy.
Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales
|January 1, 1988
Abstract:
To examine the expression of human placental lactogen, we investigated transcription in isolated placental nuclei. Using SH-agarose affinity chromatography, we have isolated Hg-RNA synthetized in vitro. These RNA are translated in a wheat germ cell-free system. hPL synthetized in vitro, accounting for 20% of total protein. This system should prove useful for the study of gene expression of hPL.