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Inhibition of VEGF gene expression in osteoblast cells by different NSAIDs
Francisco Javier Manzano-Moreno1, Victor Javier Costela-Ruiz2, Lucía Melguizo-Rodríguez3
1Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs. Granada, Spain.
Objective:
To determine the effect of different nonsteroidal anti-inflammatory drugs (NSAIDs) on vascular endothelial growth factor (VEGF) gene expression in two osteoblast cell populations.
Design:
Osteoblasts obtained by primary culture (HOp) and human osteosarcoma cell line MG63 (MG-63), which were treated with 10 μM doses of acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen or piroxicam. At 24 h of treatment, their gene expression of VEGF was evaluated by real-time polymerase chain reaction (RT-PCR) and compared with the expression in untreated cells (control group).
Results:
The treatment with the different NSAIDs significantly reduced VEGF expression regardless of the cell line and NSAID studied.
Conclusion:
The results of this study suggest that these drugs may have undesirable effects on the osteoblast and its bone-forming capacity, given the effect of this growth factor on these cells. Further studies are warranted to determine their repercussions on bone tissue and to elucidate the cell signaling mechanism/s involved.
Insights
Nonsteroidal anti-inflammatory drugs (NSAIDs) significantly decrease vascular endothelial growth factor (VEGF) gene expression in osteoblast cells. This finding suggests potential negative impacts on bone formation and osteoblast function.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- Vascular Endothelial Growth Factor (VEGF) plays a crucial role in osteoblast function and bone formation.
- Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) are commonly used for pain and inflammation, but their effects on bone cells require further investigation.
Purpose of the Study:
- To investigate the impact of various NSAIDs on VEGF gene expression in two distinct osteoblast cell models.
- To assess potential adverse effects of NSAIDs on bone-forming cells.
Main Methods:
- Primary human osteoblasts (HOp) and the MG63 osteosarcoma cell line were treated with 10 μM doses of common NSAIDs.
- VEGF gene expression was quantified using real-time polymerase chain reaction (RT-PCR) and compared to control groups.
Main Results:
- All tested NSAIDs significantly reduced VEGF gene expression in both osteoblast cell types.
- The observed reduction in VEGF expression was consistent across different NSAIDs and cell lines.
Conclusions:
- The study indicates that NSAIDs may negatively affect osteoblast function and bone-forming capacity by downregulating VEGF.
- Further research is necessary to understand the clinical implications for bone tissue and the underlying molecular mechanisms.
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