A new tool for detection of extracellular traps

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Updated: Feb 10, 2026

Neutrophil Extracellular Traps: How to Generate and Visualize Them
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Neutrophil Extracellular Traps: How to Generate and Visualize Them

Published on: February 24, 2010

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Alizé Proust1, Julie-Christine Lévesque2, Corinne Barat1

  • 1Axe des Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Québec-Université Laval, Pavillon CHUL, Québec (QC), G1V 4G2, Canada.

Methods and Applications in Fluorescence
|May 17, 2018
PubMed

Extracellular traps ejected by various immune cells (neutrophils, macrophages, eosinophils and mast cells) have several immune functions, either protective against pathogens or deleterious in some autoimmune or inflammatory disorders. Since their first description in 2004, the mechanisms of extracellular traps formation have been extensively investigated though still not fully understood. We describe here a new tool for the detection of extracellular traps by fluorescence microscopy in a single-step staining protocol, which does not require any wash. The approach uses the GreenGlo™ DNA dye, which can differentiate between nuclear DNA and extracellular DNA (extracellular traps) released from cells using different fluorescence excitation wavelengths. GreenGlo™ staining is suitable for adherent and non-adherent cells and is expected to be extendable to extracellular traps from other cells types (i.e. eosinophils, mast cells and monocytes).

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